Spotlight 59™ Oncology Panel - Ultra-sensitive somatic mutation detection from liquid biopsy samples
Designed specifically for detection of mutations from liquid biopsy samples, the Spotlight 59 Oncology Panel is a turnkey solution for Illumina sequencers, offering ultra-sensitive variant detection from low input quantities of DNA. The high sensitivity of Spotlight 59 makes it ideal for CTCs, cfDNA, and other challenging sample types such as FFPE. Combined with ERASE-Seq, Fluxion’s statistically powered variant caller, Spotlight 59 delivers 100% specificity at 0.2% allele frequency (no false positives across 27.5kb panel).
Detection to 0.1% allele frequency and below
No false positives across 27.5kb panel (average specificity >99.999%)
Covers 59 oncogenes with thousands of COSMIC mutations
Ready-to-sequence libraries in just 2 hours
Starting input quantities as low as 200 cells (including background cells), or 20 ng DNA
Average amplicon size of 140 bp for compatibility with cfDNA and FFPE
No molecular barcodes (unique molecular IDs) required; increases sensitivity, reduces bias and assay complexity
ERASE-Seq data analysis included with kit purchase
Custom panels available
IsoFlux CTC NGS Prep Kit
Designed for processing of IsoFlux CTC samples, the NGS Prep Kit prepares the recovered CTCs for genomic analysis. The kit contains purity enhancement columns and WGA reagents, producing DNA ready for NGS library prep or PCR. Used in conjunction with Fluxion’s Spotlight 59 Oncology Panel, the NGS Prep Kit creates a complete sample-to-answer assay for high-sensitivity mutation detection of solid tumor cancers from CTCs.
- Purity enhancement to 5-25% tumor DNA content increases NGS detection sensitivity
- Includes all reagents for enrichment, lysis, and whole-genome amplification
- Generates sufficient DNA for multiple NGS runs, with a starting sample as small as 200 cells (including CTCs and background)