Spotlight 59™  Oncology Panel - Ultra-sensitive somatic mutation detection from liquid biopsy samples

Designed specifically for detection of mutations from liquid biopsy samples, the Spotlight 59 Oncology Panel is a turnkey solution for Illumina sequencers, offering ultra-sensitive variant detection from low input quantities of DNA. The high sensitivity of Spotlight 59 makes it ideal for CTCs, cfDNA, and other challenging sample types such as FFPE. Incorporating ERASE-Seq, Fluxion’s statistically powered variant caller, Spotlight 59 delivers unrivaled sensitivity in liquid biopsies.

  • Detection to <0.05% allele frequency

  • Average specificity >99.999%

  • Covers 59 oncogenes with thousands of COSMIC mutations

  • Ready-to-sequence libraries in just 2 hours

  • Starting input quantities as low as 200 cells (including background cells), or 20 ng DNA

  • Average amplicon size of 140 bp for compatibility with cfDNA and FFPE

  • No molecular barcodes (unique molecular IDs) required; increases sensitivity, reduces bias and assay complexity

  • ERASE-Seq data analysis included with kit purchase

  • Custom panels available