Sensitivity, Specificity, and Low Cost: Keys for the Clinical Implementation of CTCs Detection
Speakers: María Jose Serrano, PhD, Hospital Universitario Virgen de las Nieves, and...
At Fluxion, we’re passionate about delivering cell-based solutions that facilitate the transformation of research discoveries into new ways to diagnose and treat patients. By characterizing molecular and cellular mechanisms of disease, Fluxion’s platforms help bridge the translational medicine gap, enabling rapid advances in disease research, drug discovery, and the development of diagnostic tests.
Designed specifically for detection of mutations from liquid biopsy samples, Spotlight Oncology Panels are turnkey solutions for Illumina sequencers, offering ultra-sensitive variant detection from low input quantities of DNA. The high sensitivity of Spotlight panels makes them ideal for cfDNA and CTCs.
Designed specifically for detection of somatic mutations from liquid biopsy samples, the Spotlight 59 Oncology Panel is a turnkey solution for Illumina sequencers, offering ultra-sensitive variant detection from low input quantities of DNA. Spotlight 59 is a pan-cancer panel covering 59 relevant genes. The high sensitivity of Spotlight 59 makes it ideal for cfDNA, CTCs, and other challenging sample types. Combined with ERASE-Seq, Fluxion’s statistically powered variant caller, Spotlight 59 delivers >90% sensitivity and <0.1 false positives at 0.1% allele frequency. Compatible with cfDNA and CTCs from Fluxion’s IsoFlux Liquid Biopsy System, Spotlight 59 creates a complete sample-to-answer assay for high sensitivity mutation detection of solid tumor cancers from blood samples.
59 gene oncology panel for Illumina sequencers
Complete kit that includes primers and indexed sequencing adapters. High sensitivity (2 reaction tubes per sample)
Processes 24 samples
Includes ERASE-Seq desktop application and cloud-based data analysis
The Spotlight 59 Oncology Panel offers comprehensive and hotspot coverage of 59 clinically-relevant oncology-related genes, using a 277-amplicon design to generate multiplex libraries compatible with Illumina sequencing platforms.
The Spotlight TP53 liquid biopsy NGS panel contains 21 amplicons with an average size of 140 bp that provides comprehensive coverage of all coding regions of TP53. The Spotlight TP53 Panel is compatible with short DNA fragments from cfDNA and FFPE samples. This product is a complete kit that includes all elements necessary for generating ready-to-sequence libraries, including primer pairs and indexed sequencing adapters. Combined with ERASE-Seq, Fluxion’s statistically powered variant caller, Spotlight TP53 delivers >90% sensitivity and <0.1 false positives at 0.1% allele frequency. Compatible with cfDNA and CTCs from Fluxion’s IsoFlux Liquid Biopsy System, Spotlight TP53 creates a complete sample-to-answer assay for high sensitivity mutation detection of solid tumor cancers from blood samples.
Panel with complete coverage of TP53 coding regions for Illumina sequencers
Complete kit that includes primers and indexed sequencing adapters. High sensitivity (2 reaction tubes per sample)
Processes 24 samples
Includes cloud-based ERASE-Seq data analysis
Spotlight TP53 is powered by ERASE-Seq, Fluxion’s statistically powered variant caller that is available free of charge to Spotlight users. ERASE-Seq utilizes statistical analysis to compare sequencing results to a detailed background model and technical replicates. This approach eliminates both random errors and systemic biases inherent in all sequencing workflows.
The Spotlight EGFR Liquid Biopsy Panel offers contiguous coverage of EGFR and hotspot coverage of BRAF, KRAS, and NRAS, using a 17-amplicon design to generate multiplex libraries compatible with Illumina sequencing platforms. The Spotlight EGFR Pathway Panel is compatible with short DNA fragments from both cfDNA and FFPE samples. This panel is especially well-suited for research focused on detecting clinically-relevant allele variants in DNA from cell-free DNA (cfDNA) and circulating tumor cells (CTCs).
Panel with continuous coverage of EGFR and hotspot coverage of BRAF, KRAS, and NRAS for Illumina sequencers
Complete kit that includes primers and indexed sequencing adapters. High sensitivity (2 reaction tubes per sample)
Processes 24 samples
Includes cloud-based ERASE-Seq data analysis
The Spotlight EGFR Liquid Biopsy Panel is a complete kit that includes all elements necessary for generating ready-to-sequence libraries, including primer pairs and indexed sequencing adapters for Illumina sequencers. Combined with ERASE-Seq, Fluxion’s statistically powered variant caller, Spotlight EGFR delivers >90% sensitivity and <0.1 false positives at 0.1% allele frequency.
The Spotlight Myeloid Panel combines content from peer-reviewed publications and other sources to offer comprehensive and exon-level hotspot coverage of 23 clinically-relevant genes implicated in acute myeloid leukemia (AML), myelodysplastic syndrome (MDS) and myeloproliferative neoplasms (MPN). Because of the high prevalence of myeloid variants in clonal hematopoiesis of indeterminate potential (CHIP) studies, this is an ideal panel for uncovering CH variants in either cell-derived or cell-free DNA samples.
23 gene myeloid liquid biopsy panel for Illumina sequencers
Complete kit that includes primers and indexed sequencing adapters
Processes 24 samples
Includes cloud-based ERASE-Seq data analysis
The Spotlight Myeloid Panel offers robust performance on cfDNA and FFPE samples starting with as little as 20 ng input. Compatible with all Illumina platforms, the panel has highly sensitive detection of rare variants down to 0.1% allele frequency, with sequence ready libraries in just 2 hours. Fluxion offers streamlined, free analysis of the panel via Fluxion’s ERASE-Seq Variant Caller.
Designed specifically for detection of somatic mutations from liquid biopsy samples, Spotlight custom panels are turnkey solutions for Illumina sequencers, offering ultra-sensitive variant detection from low input quantities of DNA. The high sensitivity of Spotlight custom panels make them ideal for cfDNA, CTCs, and other challenging sample types. Combined with ERASE-Seq, Fluxion’s statistically powered variant caller, Spotlight custom panels deliver >90% sensitivity and <0.1 false positives at 0.1% allele frequency. Compatible with cfDNA and CTCs from Fluxion’s IsoFlux Liquid Biopsy System, Spotlight custom panels create a complete sample-to-answer assay for high sensitivity mutation detection of solid tumor cancers from blood samples.
Fluxion offers customized Spotlight Panels optimized to enable fast, comprehensive targeted sequencing. Combined with ERASE-Seq bioinformatics, this unique approach provides a powerful solution for detecting ultra-low frequency somatic mutations using low-input DNA from cfDNA. To get started with a custom Spotlight panel, fill out the custom panel design form and submit to info@fluxionbio.com.
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Speakers: María Jose Serrano, PhD, Hospital Universitario Virgen de las Nieves, and...
Speakers: Jeff Jensen, CEO, Fluxion Biosciences
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Speakers: Mercedes Marín-Aguilera, PhD, Research Fellow, Institut d'Investigacions...