Our CTO, Dr. Cristian Ionescu-Zanetti, presented this webinar on Thursday, August 1. The webinar recording is now available at https://attendee.gotowebinar.com/register/7692209037115233805.

Webinar overview

Low signal-to-noise cfDNA-based liquid biopsies require improvements in sensitivity and specificity to provide the level of performance required for clinical utility. A common solution is the use of unique molecular identifiers (UMIs) to track individual molecules being amplified, but UMIs have their own limitations when used with real-world low DNA input samples.

This webinar will introduce ERASE-Seq, a novel approach to  accurate calling for ultra-low MAF variants that does not require the use of UMIs.

The webinar will cover the following topics:

-The cfDNA analytical challenge- how low do we need to go to achieve traditional biopsy sensitivity?
-Sources of error in liquid biopsy variant calling
-Approaches to ultra-low MAF detection - LoFreq, UMIs, etc.
-ERASE-Seq variant calling using Molecular Amplification Pools (MAPs) without UMIs
-Adaption of ERASE-Seq to existing and custom panels
-How to access and run the ERASE-Seq caller via a free trial

The following case studies will be presented:

-Applying ERASE-Seq to existing datasets
-Using ERASE-Seq with your current panel for background correction or using MAPs
-Applying ERASE-Seq to existing pre-validated NGS panels
-Development and validation of a new NGS panel

Jeff Jensen