ERASE-Seq Variant Caller: Data Sheet
Designed specifically for detection of mutations from liquid biopsy samples (ctDNA, CTCs), ERASE- Seq is a bioinformatics software solution that provides ultra-sensitive variant detection from sequencing data sets, including off-the-shelf and custom targeted panels. Currently validated on three commercial panels (Spotlight 59, Swift 56G, and Illumina TruSight Tumor 15), ERASE-Seq can be applied to any existing or custom-developed panel. Custom panels are easily validated via Fluxion’s ERASE-Seq Validation Program (EVP).
ERASE-Seq can be adapted to the desired level of detection sensitivity, DNA input, or sequencing data limits. A validated panel run in “standard” 2-replicate mode will deliver 90% sensitivity at 0.1-0.2% AF depending on the variant, with a false positive rate below 0.1 FP per sample. ERASE-Seq significantly outperforms typical molecular barcode approaches, which achieve 80% sensitivity and contain 3-5 SNV and 5-10 indel false positive calls at 0.5% AF. ERASE-Seq is also easily configurable to focus on specific variant targets to further improve sensitivity and specificity.