Spotlight 59 Panel: Instructions for Use
The Spotlight 59 Panel for Illumina platforms enables the preparation of high quality targeted Next Generation Sequencing (NGS) libraries from a variety of liquid biopsy sample types, including CTCs (circulating tumor cells) enriched from peripheral blood, and circulating, and cell-free DNA (cfDNA). For formalin-fixed, paraffin-embedded tissue (FFPE) samples, the sensitivity/specificity will be lower due to DNA damage, and computational workflow changes are needed for best performance. Please contact Fluxion for more information. Adapters are included for dual indexing and multiplexing 24 samples (kit 910-0113) or 48 samples (kit 910-0118).
The kit utilizes Illumina-compatible adapter sequences and has been validated on Illumina platforms only.