Spotlight 59 Data Sheet


Designed specifically for detection of mutations from liquid biopsy samples, the Spotlight 59 Oncology Panel is a turnkey solution for Illumina sequencers, offering ultra-sensitive variant detection from low input quantities of DNA. The high sensitivity of Spotlight 59 makes it ideal for ctDNA, CTCs, and other challenging sample types. Combined with ERASE-Seq, Fluxion’s statistically powered variant caller, Spotlight 59 delivers >90% sensitivity and <0.1 false positives at 0.05-0.1% allele frequency. Compatible with ctDNA and CTCs from Fluxion’s IsoFlux Liquid Biopsy System, Spotlight 59 creates a complete sample-to-answer assay for high sensitivity mutation detection of solid tumor cancers from blood samples.

[Download PDF]