KRAS/BRAF mutation detection via qPCR

Overview

This document is intended to guide the user through a method for detecting point mutations on the KRAS and BRAF genes using samples collected on the IsoFlux System. This protocol utilizes a sensitive allele specific TaqMan assay with competitive blocker (castPCR®) and is detected using a standard qPCR instrument. This protocol has been validated by Fluxion using analytical and clinical samples. Please consult Fluxion for any modification to this protocol.

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ProtocolKirk JensenPCR