IsoFlux CTC Enumeration Kit

INTENDED USE

The intended use for the IsoFlux™ Circulating Tumor Cell Enumeration Kit (CTC Enumeration Kit) is as a laboratory reagent for the fluorescence staining of cells to identify circulating tumor cells (CTCs) that are cytokeratin (CK) positive, CD45 negative, and nucleated. The kit is designed for use with the IsoFlux System, a benchtop instrument for semi-automated rare cell isolation. The kit contains antibodies and reagents for immunofluorescence staining. The CTC Enumeration Kit is for Research Use Only. 

SUMMARY AND EXPLANATION

Circulating tumor cells (CTCs) are cancer cells that shed from a primary or metastatic tumor and enter the peripheral circulation. Carcinomas are cancers of epithelial origin and include breast, prostate, lung, and colorectal cancers. This document is intended to guide the user through a typical procedure of immunofluorescence staining, fluorescent microscope imaging, and image analysis to enumerate circulating tumor cells (CTCs) of epithelial origin isolated using the IsoFlux instrument. 

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