IsoFlux NGS Workflow for CTCs
Cristian Ionescu-Zanetti, PhD
Chief Technology Officer
Christopher Davies, Director of Bioinformatics, IonTorrent / Thermo Fisher
In this webinar, you will learn about a breakthrough technology for detecting somatic mutations in cancer samples using only a blood sample.
The IsoFlux System is the first instrument of its kind to capture rare circulating tumor cells (CTCs) with sufficient recovery and purity to meet the demands of Next Generation Sequencing (NGS) applications.
In this webinar, you'll see examples of how a complete workflow can be established in your own lab to track mutations in cancer samples, all using your own existing NGS pipeline.
Topics will include:
The promise of the blood biopsies in cancer management
Sample challenges for NGS applications
NGS workflow overview
Use of the AmpliSeq Cancer Hotspot Panel v2
Optimization of the variant calling and filtering in Ion Reporter
Analytical validation of CTC NGS assay
Clinical patient data using NGS assay