ERASE-Seq variant caller- superior performance to 0.1% allele frequency and below, without barcodes!
ERASE-Seq Analysis 10-pack
Finally, the promise of sensitive, accurate liquid biopsy analysis by NGS has been realized! ERASE-Seq uses inter-sample signal processing to eliminate background artifacts and random sequencing errors, all without the need for molecular barcodes. ERASE-Seq works by separating out the sources of error- systemic biases and stochastic errors- and using statistical signal processing to remove both. ERASE-Seq has been proven in sequencing of ctDNA and CTC samples, and excels in the allele frequency range of 0.05% to 0.5%, where barcode approaches lose both sensitivity and specificity.
ERASE-Seq is modular and flexible, and can be tailored based on desired performance, sequencing depth, and available DNA:
|Standard||Background Correction Only||Ultra-sensitive|
|90% Sensitivity LOD (AF)||0.1-0.2%||0.25-0.5%||0.05-0.1%|
|False Positives at LOD||0.1||0.1||0.1|
|DNA Input Requirement||20ng||10ng||40ng|
|Replicates (reaction tubes)||2||1||4|
|Overall Sequencing Depth||10,000X||5,000X||20,000X|
ERASE-Seq is the proven solution for NGS of liquid biopsy samples. Key benefits include:
Superior sensitivity and 10-100X fewer false positives than molecular barcodes (UMIs)
Excels in the 0.05%-0.5% AF range where molecular barcodes struggle
Any targeted panel can be adapted easily to ERASE-Seq with software validation- and no barcodes needed
Provides similar detection performance to digital PCR, but ERASE-Seq can test for thousands of variants per test
Customized variant target lists
Validated in numerous sequencing labs globally
ERASE-Seq can be applied to virtually any targeted sequencing panel. Currently-validated panels include Fluxion's own Spotlight 59, Illumina Trusight Tumor 15, and Swift 56G. Additional ERASE-Seq technical information and IFUs are available in our Library.
Custom Panel Development
Fluxion offers customized Spotlight Panels optimized to enable fast, comprehensive targeted sequencing. Leveraging a combination of unique design and chemistry, custom Spotlight Panels produce overlapping (tiled) amplicons in a single-tube using a rapid 2-hour workflow. Combined with ERASE-Seq bioinformatics, this unique approach provides a powerful solution for detecting ultra-low frequency somatic mutations using low input DNA from challenging samples types such ctDNA. A fast and easy single-tube workflow produces the best-in-class performance for on-target percentage and coverage uniformity, enabling low frequency variant discovery and confirmation. To get started with a custom Spotlight panel, fill out the custom panel design template and submit to firstname.lastname@example.org.